A Method for Stacking Genes into a Single Genomic Site Through Iterative Genetic Transformation of Plants



Designer plants can be made by integrating allogeneic or xenogeneic DNA into a plant population.  Those techniques have resulted in numerous commercial cultivars, and those varieties have increased yields with the use of fewer herbicides and pesticides.  Strategies using plants as bioreactors for human and animal pharmaceuticals also have been demonstrated.  Therefore, developing molecular techniques that allow desired genes to travel as a package through the plant selection process would save time and enhance future plant breeding schemes.


Transforming plants using our method of “gene stacking” or “molecular stacking”, is suitable for breeding multiple (transgene-encoded) traits in plants.  Typically, plant transformation relies on selection marker genes which enhance gene integration efficiency.  Our “clean” gene stack does not contain undesirable DNA elements such as marker genes or recombination sites. 


Insertion of multiple genes into a single locus simplifies breeding as the “stack” of genes would essentially transmit as a single locus through sexual crosses – leaving only the genes that are useful for production. Introducing the genes into a single location will make it easier to maintain all traits while performing traditional breeding for other improvements.

Provisional Patent Application has been submitted


For licensing opportunities please contact:

Technology Commercialization Office




REF: 2013-25

Patent Information:
Plant Agriculture
For Information, Contact:
Bryan Renk
Associate Director for Technology Commercialization
University of Arkansas TCO
Vibha Srivastava
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